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Not long after a Yoshi, which had been separated from the Stork and the other babies, ended up finding the lost Baby Wario and together they managed to escape the enemy filled area and they continued to aid each other throughout the rest of World 3. But Baby Wario did not remain with the Toadies for long, due to his terrible tantrums, so when the Toadies finally became fed up with the baby they abandoned him in an undersea cave in World 3–5. He along with numerous other infants had been abducted by the evil Kamek's Toadie army in order to find the legendary Star Children that were spoken of in legend to be the only thing standing in the way of the Koopa Troop's attempt at world domination. Wario's first chronological appearance was in Yoshi's Island DS.

Wario went on to become the game's main antagonist by taking over Mario's castle, which met the staff team's initial vision of diverting from the conventional objectives established by previous Mario games. To Kiyotake's surprise, the idea to flip the 'M' seen on Mario's Cap to create a 'W' received extremely enthusiastic support by the rest of the staff. Kiyotake subsequently discussed his idea with his assistant character designer and co-director Takehiko Hosokawa, with whom Kiyotake normally sought approval from before presenting an idea to the rest of the game's staff.

Bluto is physically well-built, motivated by self-interests, and more cunning than his counterpart, Popeye. Kiyotake stated that Wario's relationship to Mario was inspired by the American comic book characters Popeye and Bluto. Wario's physical design and personality was based on the idea that a protagonist should have an arch-rival and nemesis. Before any actual character development, Wario's name was the first thing that was decided on, being derived from the Japanese word 'warui' (悪い), meaning 'bad'. It was initially director Hiroji Kiyotake's idea to introduce Wario as a new character that fits this change in objective, although several rejected characters were presented earlier which no one took a liking to. Super Mario Land 2: 6 Golden Coins official Shogakukan game guide, staff interview on pages 106-111 ( translation provided by 'Shmuplations', accessed September 10, 2016) Rather than having Mario fight for the benefit of someone else (such as Princess Daisy in Super Mario Land 2's precursor, and Princess Peach throughout other games), the staff wanted Mario Bros to fight to win back something of his own. The staff team aimed for a 'change of pace', and to not be bound by the ideas which were continuously used in Mario games leading up to that time they desired to provide Mario with a new objective. The idea to introduce Wario as a new! Italic character originated during development of Super Mario Land 2: 6 Golden Coins.

Super mario strikers charged daisy voice lines